Ion semiconductor sequencing
Has developed a system based to using standard sequencing chemistry, (Life Technologies, Inc. is now owned) in the detection system of new semiconductor-based Ion Torrent Systems, Inc.. In contrast for sequencing, and an optical method that is used in other systems, this method of sequencing is based on the detection of the hydrogen ions released during the polymerization of DNA. Chain DNA template consisting of microplate was determined the nucleotide sequence of nucleotides types are flooded. Base leading template nucleotide complementary is incorporated into the growing complementary strand when it is introduced. This leads to release to induce indicating that the reaction has occurred, the hydrogen ions hypersensitivity ion sensor. If present in the template sequence repeat homopolymers, nucleotides includes multiple cycles. This results in a corresponding number of hydrogen atoms, which has announced a higher proportional electrical signal.
Ion semiconductor array is a method for DNA sequencing based on the detection of the hydrogen ions released during the polymerization of DNA. While the complementary strand is built based on the sequence of the template strand, which is a method of “sequencing-by-synthesis”. Chain DNA template consisting of microplate was determined the nucleotide sequence of one deoxyribonucleotide triphosphates of (a dNTP) is flooded. If it is incorporated into the complementary strand template nucleotides leading complementary dNTP was introduced is growing. This leads to the release of hydrogen ions which indicates that the reaction has occurred to trigger the ISFET ion sensor. If present in the template sequence repeat homopolymer, dNTP molecule contains more than one cycle. This results in a corresponding number of hydrogen atoms, which has announced a higher proportional electrical signal.
This technology is different from the other techniques for sequencing and to use nucleotide optical system, has not changed. Further ionic semiconductor sequences mediated sequence SEQ silicon ion flux, the pH, or it may be a sequence in a semiconductor. DNA Electronics Co., Ltd., which was developed by Ion Torrent Systems, has been licensed from the Corporation, the technology, was released in February 2010. Can be as a desktop machine, to be used in many laboratories, commercial ion Trent machine sequencer as economical high-speed, compact. The Roche 454 Life Sciences DNA Electronics partner reading for a long time, in the development of high-density semiconductor sequencing platform that uses this technology.
In nature, (dNTP a) include deoxyribonucleotide triphosphates with growing chain of DNA involves the formation of hydrogen release positively charged ions with pyrophosphate and covalent bond. It contains dNTP when it is complementary to the tip template nucleotide pair. In order to establish hydrogen ions whether the release delivery of dNTP kind of reaction, ion semiconductor sequencing, uses these facts. Be full unmodified sequentially microplate DNA polymerase and a semiconductor chip that contains a single-stranded DNA template molecule to be sequenced, respectively, with the dNTP T C, or G,. Complementary pairs of nucleotides template strand, is incorporated into the growing complementary strand of DNA polymerase if you enter the next dNTP. Of dNTP complementary was introduced included, if it is not biochemical reaction. The pH detected by the ISFET, hydrogen ions are released in the reaction of the solution changes. DNTP from molecules that are not attached is cleaned before the next cycle of dNTP different types is introduced.
I have an ion-sensitive layer beneath is a ISFET ion sensor under the well layer. CMOS semiconductor chips similar to those used in the electronics industry, all layers are included within. Each chip contains a number of wells and ISFET of the detector. Then, hydrogen ions are released each leads to ISFET ion sensor. The events associated with signal for incorporation of nucleotides, the sequence of electrical pulses sent from computer chips, optical measurement and nucleotides consists of DNA sequence without intermediate conversion was measured directly the electronic device, it is labeled use of is avoided. It can be implemented in software assembly and DNA signal processing. Precision reached base House Trent Trent ion ion ion semiconductor sequencer, in February 2011, was 99.6%, based on the 50 reading 100 MB of each execution. The length of the reading of February 2011 was 100 base pairs. Accuracy was 98% of the repeating homopolymer length of five replicates. It should be noted that the data is still independent, it is not validated external