Sense and antisense

Sense and antisense is part of the building blocks of the DNA double helix, base pairs contributes to the interior structure of RNA and DNA. In order to maintain the helical structure of the constant that is independent of the nucleotide sequence, the hydrogen bonds to allow the DNA strand, Watson – is determined by the specific pattern (thymine – – adenine and cytosine guanine) Crick base pairs. Complementary nature of the base pair structure provides a backup copy of all genetic information encoded in double-stranded DNA. Data redundancy and regular structure provided by the double helix DNA DNA, which has been made suitable for the storage of genetic information, base pairing received between nucleotides and DNA, the sequence of the DNA polymerase opposite strand is, I called “antisense” sequence. Different parts of the same strand of DNA (i.e., including the sense and antisense sequences to the two chains) sense and antisense sequences may be present in the. In prokaryotes and eukaryotes, to produce an antisense RNA sequence, RNA functions these are not entirely clear. One proposal is that it is involved in the regulation of gene expression through base pair RNA-RNA antisense RNA.

Illustation of the Sense and antisense properties of DNA

Sequences of prokaryotes and eukaryotes of DNA some more and more and a virus or plasmid, blurs the distinction between the sense and antisense strands by having a gene overlap. In these cases, the DNA sequences of several double duty encoding the protein molecules leading of one strand, and performs a second protein to be read in the opposite direction along the other strand. In bacteria, this overlap may be involved in the regulation of gene transcription while increasing the amount of information that can be viruses, gene duplication is encoded in a small viral genome.

The molecular biologist, call the chain (or plus (+)) of the DNA sense if the version of the same RNA sequence is translated into a protein or translated. The complementary strand antisense – called () or negative sense (). Phrase coding strand occurs occasionally, by transferring in both directions from the promoter region of the common some cases that encode non-coding RNA and protein also can be transcribed from both tracks, or two (see transcription following “ambisense” is an intron in the chain).

Complementary strand of two double-stranded DNA of (dsDNA) are separated as a chain “anti-sense” and usually, “sense” strand. In the sense strand, DNA is similar to the (mRNA) RNA, the human eye (e.g., ATG codon = methionine amino acid) may be used to read the protein code that is expected. However, DNA of the sense strand itself is not used for the preparation of proteins by the cell. This is an antisense DNA strand that serves as a source of protein source used as a template the base complementary DNA strand sense mRNA. Since the transfer results in a product of the RNA strand DNA template complementary mRNA, it is complementary to the antisense DNA strand. I used to (protein synthesis) translation of this mRNA.

Therefore, it is possible to cause the sense and antisense 5′-August 3 ‘base nucleotide triplet 3’-TAC-5 of the antisense strand of DNA “(methionine August codon, the start codon) in the triplet mRNA in use as a template The. distance sense DNA is, will have the ATG triplet that look exactly the same as in August, but not to be used to make mRNA, it is the effect of DNA strands. can not be used to methionine called “sense” is, as it is used, the protein is not a spiral, it is because having a sequence similar codon of the protein sequence.

Illustration of the Sense and antisense DNA property

If you do not understand the meaning of “meaning” They are, if you do not understand the complementarity, it might be confusing to students they. before the treaty is established, in order to further confusion, things are “nonsense” does not agree to the DNA strand, called “sense”, the oldest textbooks. In biological research, short antisense molecules may be reacted alter the expression of genes with a complementary strand of nucleic acid. Please see the following the “antisense oligonucleotide”.

DNA can be used to twist the rope in a process called DNA supercoiling. DNA and is a “relaxed” state, in the case of the DNA, twist strands become more tightly around the axis of the double helix, loosely wrapped once more usually every 10.4 base pairs chain. If it is twisted in the direction of the spiral, which is a positive supercoiling, DNA, is held tightly from each other bases. They are if you are twisted in the opposite direction, this is a super-coil negative, the base dissociate easily. In nature, DNA most is supercoiled slightly lower are introduced by an enzyme called topoisomerase. These enzymes are needed to relieve the torsional stress that was introduced into the DNA strand in the process of such transfer and replication of DNA.

Antisense RNA is a RNA transcript that is complementary to the endogenous mRNA. In other words, this is a non-coding strand, in addition to the coding sequence of RNA, which is the same as the negative sense RNA viruses. Introduction of a gene encoding antisense RNA is a technique that is used to block the expression of the gene. Antisense RNA is radioactively labeled may be used to display the transcription levels of genes in various cell types. The type of alternative part of the structure of the antisense, the antisense therapy at least one approved for use in humans treated antisense Related experimentally.

In forming a double stranded RNA and antisense sequences complementary, translation is blocked mRNA. This process is related to RNA interference. In order to prevent the expression of specific genes, antisense nucleic acid molecules can be used experimentally to bind to mRNA. Antisense therapy is under development in the U.S. Food and Drug Administration has approved phosphorothioate antisense oligo fomivirsen and (Vitravene) mipomersen the (Kynamro) for therapeutic use of human as well (FDA). Cells can be used to generate antisense RNA molecules that interact with mRNA molecules complementary naturally, to inhibit their expression. There is provided a mechanism for replicating the DNA, RNA polymerase transcription of DNA into RNA. DNA binding proteins, many can recognize a specific pattern of a pair of specific type specific regulatory region of the gene.

Intramolecular base pairing may occur in single-stranded nucleic acid. (E.g., AA or GU) to allow the various interactions of the click, Watson – – Non-Watson and formation of the double helical short chain This includes a (AS and GC) Crick base pairs, RNA molecules (e.g. , RNA is particularly important in transfer RNA), can be folded into a wide range of three-dimensional structure of a particular. Further, between messenger RNA base pairing transcribed RNA and the (tRNA) and (mRNA), as a result of the base sequence of mRNA underlying molecular recognition events, was translated into the amino acid sequence of the protein.

The DNA, because it is a double-stranded Generally, in many cases, the size of the entire genome of an organism or individual genes is measured in base pairs. Therefore, the number of base pairs in all is the number of nucleotides in (excluding non-coding region of one strand telomere) one of the wires. Estimates (23 chromosomes) long and about 3.2 billion base pairs in the haploid human genome, and contains the different genes from 25.000 to 20.000. Kilobases (KB) is a unit of molecular biology equal to 1000 base pairs of RNA or DNA.

In the double helix of DNA, each type of nucleic acid base of base 1 chain link on the only other circuits. This is called an auxiliary base pairs. Here, purines form hydrogen bonds thymine, adenine, pyrimidine gluing guanine cytosine bonded by hydrogen bonds only three hydrogen bonds and only two. This arrangement of two nucleotide binding, is called base pairs in the double helix. Hydrogen bond is not a covalent bond, it is possible to separate them, and recombine relatively easily. Both strands of the DNA double helix, you can zip by, or to open by high temperature and mechanical forces. As a result of this complementarity, all information in the helices of the two-stranded DNA sequence repeats every section is essential for DNA replication. Indeed, a specific interaction with this reversible, is important for all functions of DNA in living organisms between complementary base pairs.