In molecular biology, it offers a lot of small nuclear RNA 7SK found in metazoans. By controlling the transcription elongation factor P-TEFb positive, it plays a role in the regulation of transcription. The 7SK, has a (human) nuclear ribonucleoprotein complex small number of other proteins which modulate the function and stability of the complex. In early studies, the number of 7SK proteins to cells, and have demonstrated that it comprises a study of secondary structure model proposed for the pair on the basis of the different regions of the RNA. Transcription elongation factor P-TEFb of breakthrough positive that comes with the knowledge that it is a component of the complex in the function of 7SK human origin. Binds to 7SK is, to inhibit the activity of cyclin P-TEFb by the action of HEXIM1 HEXIM2 or RNA-binding protein. The 5 ‘end of 7SK methylation methylphosphate ceiling MEPCE enzyme is a component of the 7SK human γ phosphorus. The part may be due to interaction with the 3 ‘end of the RNA also found LARP7 fusion proteins la, it is associated with a 7SK. Reducing the LARP7 or MEPCE knockdown by siRNA leads to destabilization of 7SK in in vivo. Rather than lack HEXIM and P-TEFb, 7SK snRNPs in some hnRNPs are included.
The main function of the factors regulating the elongation phase 7SK human control P-TEFb, a transcription. Kinase activity when P-TEFb coefficient of 7SK human origin is inhibited. You can release including P-TEFb, a protein BRD4, a bromodomain from HIV or beta Tat or 7SK human origin. In this release, result in structural changes in the discard HEXIM 7SK RNA. Stabilization hnRNPs lacks the HEXIM and P-TEFb complexes. The P-TEFb function after gene re-isolated 7SK people from a particular by an unknown mechanism. I is characterized by both the Drosophila and human human 7SK.
Human 7SK RNA is a generation rich 331 NT nuclear transcription by RNA polymerase III. Join HEXIM1 / 2 of 7SK RNA is the protein inhibitor of these P-TEFb of (positive elongation transcription factor B). P-TEFb is required for transcription elongation of RNA polymerase II. Leads to the activation of P-TEFb, retention and transfer, 7SK RNA is released from between P-TEFb/HEXIM/7SK complex physiological stimuli such as cardiac hypertrophy. Subset of (hnRNP of) Freed 7SK RNA fellow heterogeneous nuclear ribonucleoprotein. 7SK RNA, which is stored in vertebrate evolution, the same family has been found worms, insects and mollusks genome. The folding pin 7SK RNA several serving as a platform for the specific binding protein. It is stabilized by end monomethylated ‘La related binding proteins, LARP7 its three and three phosphate groups 5’ thereof. Characterized to regulate transcription of ncRNA paradigm best 7SK RNA probably.
Deletion analysis was performed human 7SK RNA gene, the contour of the major areas in vitro transcription by RNA polymerase III. The 37 above between 3 from BP 7SK RNA coding region there is a sequence promote transcription. RNA polymerase III promoter flanking sequences plasmid transcription, it can be the supervision shows that the internal promoter sequence is not required, a complete absence of 7SK RNA coding region. Transcription, to stretch feature the end of the T residues of RNA polymerase III transcription. Reduce the efficiency TATA, of significant transfer box, at position -25, organizer, has a mutation in it. Upstream sequence, enhance the efficiency of the organizer to -243 from position -37. Promoter recognized by RNA polymerase III is similar to the promoter of gene transcription by RNA polymerase II functionally also structural.