Antisense RNA

Antisense RNA (asRNA) are single-stranded RNA complementary to RNA transcribed in the cell circuit (the mRNA). I use (non-interference of the mRNA complementary RNA), but some authors, not widely used long-term micRNA to refer to these RNA. Antisense RNA may be introduced into cells to inhibit the translation of mRNA of the auxiliary base pair with it, interfering with translation mechanism physically. Therefore, this effect is stoichiometric. Examples of occurrence, mechanism antisense mRNA was plasmid NOK / juice system EE R1 course. It is considered a promising method for the antisense RNA to treat a long disease, if only reached the market, some drug fomivirsen. “10 technology is a great concept, irritating. To [commercial] a” common feature of anti-sense RNA, an effective design, antisense RNA is an effective administrative and biological activity as one of the One commentator No route.

Historically, usually by targeting the RNA-induced silencing complex, fragments of double-stranded RNA, called small interfering RNA mediates silencing of the gene responsible for the catalyst often influence of antisense RNA is digested by binding is disrupted by the impact of (RNAi) RNA interference is related to a process (RISC) mRNA. Attempts to genetically engineered transgenic plants expressing the antisense RNA rather than, although the process will lead to different sizes of the same effect on gene silencing downstream activates the RNAi pathway. There are two types of papaya ringspot resistant and Flavr · Saber tomato well-known example.

Long cis – transcription of the antisense transcript is a phenomenon common to transcriptome in mammals. Functions in some cases have been described as Zeb2/Sip1 antisense RNA, but the overall functionality is not yet known. For Zeb2/Sip1, [8] non-coding antisense RNA to the 5 ‘splice site of intron 5’UTR of ZEB2 mRNA. Expression of non-coding antisense prevents the splicing of introns, including the ribosome entry required for efficient expression of the protein ZEB2. In many cases, a long antisense RNA, transcription of antisense, do not agree with genes encoding related proteins, but further investigation pattern relative expression of antisense non-coding mRNA and is complicated It has been shown.

Antisense transcription unit of natural (NAT) is a group of RNA encoded within the cell for transcription complementarity with other RNA transcription. They have been identified in eukaryotic organisms including some Arabidopsis human, mouse, and yeast. It includes both non-coding RNA and protein-coding of this class RNA. This data can be the role of various regulations, RNA interference (RNAi), alternative splicing, to X-chromosome inactivation and genomic printed by this technology. And based on whether or not to act in cis or trans to the rough, NATs’s can be divided into two categories. It is transcribed from elsewhere than its goal, NAT of the transformer, has multiple copies of the supplements and some contradictions usually. The micro RNA (the miRNA), is an example of a transaction that can turn copy NATS and several discrepancies some. Rather than from the same genomic site that purpose, transcribed from DNA strand opposite cis natural antisense transcript (cis NAT), so as to form a perfect pair on the other hand.

The length of the overlap between the pairs and different orientations have different NATs – cis. There are five guidelines to identify the NAT of the Sith to date. Includes two transcripts together 5 ‘end, the most frequent and posture of the head to head. Lead to knock down the largest of gene expression in transcription orientation collision reason look up suppression such. However, tail shows that there is some research – the direction of the tail overlap, as the tail of the tail, NAT. Near the tail, it is less common to tail in pairs the most common other, and close to the head-to-head. On top of each other to include antisense gene is full, head near complete duplication NATs – head and tail – the direction of the tail are physically separated from one another, but are very close to each other. I have shown that there is overrepresentation of NAT pair of genes data has a catalytic activity. In particular can be, it becomes more susceptible to them for this type of adjustment, this has something to these genes.

Identification of NAT of all genome, is possible thanks to a large collection of sequence data available from different organisms. Depending on the source of sequence information, a method for detecting the NAT on in silico suffer from several drawbacks. research is mRNA sequence orientation is known, using the amount of available mRNA sequence information is small. Model was estimated using the training genetic algorithm to determine a gene, provides coverage with increased genome of the cost of the gene in trust. Another input is a library (EST) wide expressed sequence tagging, but before you can extract from it useful information orientation, you must first determine a small sequence of these. If you are using the EST special sequence information, such as splice sites for both filters EST poly (A) signal, and a poly A tail, Several studies have obtained the right direction of transcription them. increase the coverage, a combination of the source sequence that differs, trying to maintain the integrity of the data.

Pairs of NATs when forming the clusters they overlap are identified. In general, ~ is considered the transcript of 20 nucleotides of sequence overlap, usage restrictions in research volatility variety there is, is considered the minimum for the cluster overlap. Further, as can be regarded NAT pair of copying correlates with mRNA molecules of each other it. It can be used to find antisense pairs Currently there are various resources or software web. Database antisense transcript or natural NATsdb is a rich search tool meaningless pair of various organisms